Construction and evaluation of introgression lines and fine mapping of ehd8 from Jinghong common wild rice (Oryza rufipogon)

Heading date is one of the most important traits in rice and regulated by multiple genes. Common wild rice is the ancestor of Asian cultivated rice and harbours abundant genetic diversity. To use wild rice resource in rice breeding, a set of 154 introgression lines (ILs) covering 93% of the genome of Jinghong common wild rice was constructed in the background 'Teqing', using 208 simple sequence repeat markers evenly distributed on 12 chromosomes. Among the ILs, the line JIL64 displayed late heading independent of photoperiod. Genetic analysis using the two F₂ populations crossed ''Teqing'/JIL64 and JIL64/'Teqing' revealed that late flowering was controlled by a recessive gene on chromosome 8 (designated early heading date 8, ehd8), and ehd8 was fine mapped to the 50‐kb region flanked by markers RM22221 and 64Indel4. Sequencing and qRT‐PCR demonstrated that LOC_Os08g01410 and LOC_Os08g01420 were deleted in JIL64 and may be associated with the late heading of Jinghong common wild rice. These findings lay a practical foundation for characterizing ehd8, and the ILs help to mine genes from Jinghong common wild rice.     

完善内地主产棉区棉花补贴机制的思考与建议——以山东省为例

总结了棉花目标价格补贴政策实施以来山东植棉区的实施进展、成效与问题。从国家棉花产业安全和山东省经济社会发展角度，分析了山东植棉区棉花生产的重要地位和保持补贴政策稳定性的重要意义。以山东为例，提出了内地主产棉区棉花补贴政策改革建议。     

High water content silk protein-based hydrogels with tunable elasticity fabricated via a Ru(II) mediated photochemical cross-linking method

Silk is very promising in the field of biomaterials as a natural biomacromolecule. Silk protein can be made into various forms of materials, including hydrogels. However, silk protein-based hydrogels have not attracted much attention due to its weak mechanical properties. Here, we report high water content silk protein-based hydrogels with tunable elasticity which were fabricated through Ru(II) mediated photochemically cross-linking tyrosine residues in regenerated silk protein. The regenerated silk protein was characterized by Fourier transform infrared spectroscopy (FTIR). The gelation kinetics of the silk protein was studied by rheology measurements. The compressive mechanical properties of the silk protein-based hydrogels was investigated using compressive tests and dynamic mechanical analysis (DMA). Compressive modulus of the hydrogels reached 349±64 MPa at 15 % strain. The fabricated silk protein-based hydrogels were also characterized by Scanning electron microscopy (SEM), revealing an interconnected porous network structure, typical of hydrogels, with an average pore size of approximately 130 μm. Finally, biocompatibility of the silk protein-based hydrogels was demonstrated through cell culture studies using a human fibroblast cell line, HFL1. The reported silk protein-based hydrogels represent a promising candidate for biomaterial applications.     

棉花“保险+期货”模式助力南疆主产棉区脱贫攻坚

通过解析棉花“保险+期货”模式的运行机制、优势及存在的不足，并提出了发展优质棉“订单生产+保险+期货”模式和引入银行、基金等信贷机构的建议，期望对南疆主产棉区贫困棉农增收致富、助力脱贫攻坚提供有益探索。     

体细胞克隆荷斯坦奶牛出生后死亡犊牛内脏器官的组织病理学观察

为探究体细胞克隆荷斯坦奶牛出生后死亡的原因,对新生后死亡的克隆荷斯坦公牛和自然繁殖的荷斯坦公犊的主要组织器官进行比较。通过解剖和石蜡切片-HE(hematoxylin-eosin staining)染色技术,对主要的组织器官结构进行观察和分析。结果表明,新生后死亡的克隆荷斯坦公牛肺部结构清晰;肝脏的肝细胞明显肿大,出现脂肪轻度变性;肾小管上皮细胞出现变性;心肌的肌纤维间空隙增大;骨骼肌纤维间隙明显,空泡变性,这可能导致该克隆牛犊肌肉无力并功能不全;淋巴结皮髓质界限不分明,淋巴小结细胞较稀疏,生发中心不明显,淋巴窦细胞较少;脾脏内红细胞较少,这说明该克隆牛的造血功能可能不完善;胸腺的皮质部分和髓质部分界限不明显,嗜酸性胸腺小体不易辨认,可能发育不全。该克隆公牛免疫器官出现不同程度的发育不全现象较严重,这有可能是其出生后死亡率高的主要原因。     

羊支原体性肺炎两标准株对抗菌药物敏感性的研究

本实验测定了环丙沙星、氧氟沙星、单诺沙星、红霉素、罗红霉素、泰勒菌素、泰妙菌素、四环素等8种药物对羊肺炎支原体两个标准株Y98和Y-goat的体外抑菌浓度以及红霉素与氧氟沙星、泰勒菌素对Y-goat和四环素与氧氟沙星、泰勒菌素对Y98的联合药敏作用。对羊肺炎支原体两个标准株对抗菌药物的敏感性进行了分析。     

预应力薄腹梁质量事故的分析及鉴定

阐述了某工程由于施工原因，导致预应力混凝土工字形屋面梁强度不足的预制构件的检验鉴定过程和方法。采用回弹法推定混凝土实际强度；通过静载试验测试构件的实际挠度、抗裂和承载能力。分析了导致混凝土强度不足的原因。     

甜菜坏死黄脉病毒(BNYVV)75kDa通读蛋白基因及其54kDa片段的克隆与表达

 以甜菜坏死黄脉病毒(BNYVV)内蒙分离物的总RNA为模板,通过反转录和PCR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。     

常规鱼类养殖技术之五广东鲂人工繁殖技术

广东鲂是珠江水系西江著名特产之一,也是西江重要的经济鱼类,近年来,其自然资源量呈逐渐衰退之势,90年代的渔获量仅在5t左右徘徊.为此,广东省封开县水产研究所开展广东鲂人工繁养殖技术研究,笔者总结5年来的经验,介绍广东鲂人工繁殖技术如下:     

论科技情报的信息媒介意义

科技情报作为一种重要的信息媒介。作者着重地阐述了其具备的宏观俯瞰,微观入里的导引功能。就其科技情报意义与林业科研及生产领域上的信息交流而言提出了自己的见解。